藜欢解郁方中抗抑郁成分的不同提取方法比较

Comparison of Different Extraction Methods for Antidepressant Components from Lihuan Jieyu Prescription

目的:优选藜欢解郁方中抗抑郁成分的提取方法。方法:采用水提法、醇提法、水提加醇提法提取藜欢解郁方中抗抑郁有效物质,运用小鼠强迫游泳试验、敞箱试验、拮抗利血平所致体温降低试验优选提取方法。结果:在小鼠强迫游泳试验中,藜欢解郁方水提物组与模型组比较能明显缩短小鼠不动时间,表现出抗抑郁作用。拮抗利血平所致体温降低试验中,水提物组与模型组比较有显著性差异,敞箱试验表明各组间小鼠自主活动无显著性差异。结论:水提法为藜欢解郁方藜欢解郁方抗抑郁的最佳提取方法。

Objective：To optimize extraction method of antidepressant ingredients from Lihuan Jieyu prescription.Method： Water extraction,ethanol extraction and water-ethanol extraction were used to extract antidepressant active substances from Lihuan Jieyu prescription,optimum extraction method was determined by pharmacodynamics tests,such as forced swimming test,reserpine hypothermia test and open tank test in mice.Result： Compared with model group,water extraction group of Lihuan Jieyu decoction could significantly shortened immobility time of mice in forced swimming test,it showed antidepressant effect.There was significantly difference of water extraction group and model group in reserpine hypothermia test.In open tank test,there was no significant difference of spontaneous activity in all groups.Conclusion：Water extraction method was optimum extraction technology for antidepressant components from Lihuan Jieyu prescription.