超临界CO_2流体萃取法与干法熬制提取暹罗鳄鱼油的比较

Analysis of CO_2 Supercritical Fluid Extraction and Decoction Methods to Extract Crocodylus siamensis Oil

目的:分别采用超临界CO2萃取法及干法熬制提取暹罗鳄油脂,并进行油脂得率、脂肪酸得率及油脂主要成分的比较研究。方法:分别采用超临界CO2萃取法及干法熬制提取暹罗鳄油脂,比较油脂及脂肪酸得率,并对2种工艺提取的鳄鱼油脂进行GC-MS分析,面积归一化法测定计算各主要成分的相对百分含量。结果:超临界CO2萃取法、干法熬制油脂得率分别为32.6%,60.0%,脂肪酸得率分别为73.06%,76.78%,两种提取方法所得油脂的主要化学成分含量不完全相同。结论:干法熬制提取暹罗鳄鱼油在油脂得率方面有显著优势,超临界CO2萃取暹罗鳄鱼油脂的方法仍待进一步研究与优化。

Objective：CO2 supercritical fluid extraction and decoction methods were used to extract Crocodylus siamensis oil,respectively.The oil yield,fatty acid yield and main oil compositions were set to be the investigation index.Method： C.siamensis oil was extracted by CO2 supercritical fluid extraction and decoction methods respectively.The oil yield and fatty acid yield were compared.The relative percentage of the components was determined by the area normalization method using gas chromatography（GC） / electron impact（EI） mass spectrometric（MS）.Result： The oil yields were 32.6%,60%,respectively,while the fatty acid yields were 73.06%,76.78%.Mainly compositions extracted by the two methods were not identical.Conclusion： Decoction method has certain advantage over CO2 supercritical fluid extraction method at the aspect of oil yield.The way of extracting C.siamensis oil by CO2 supercritical fluid extraction method still needs further study and optimization.