射干伪品中柠檬黄与日落黄的研究

Study of Lemon Yellow and Sunset Yellow in Adulterated Rhizoma Belamcandae

目的:鉴别并测定伪品射干中水溶性色素柠檬黄与日落黄。方法:采用薄层色谱法和高效液相色谱法二极管陈列检测鉴别并定量测定样品中的柠檬黄与日落黄。结果:伪品射干中可检出柠檬黄与日落黄,HPLC法测定柠檬黄含量为0.11～0.37 g.kg-1,日落黄含量为0.04～0.11 g.kg-1。结论:以川射干或其他伪品用柠檬黄与日落黄染色作为射干销售,即无必要,又曾加临床使用不安全因素,值得相关药品研究与生产单位注意。

Objective：To identify and assay of watersoluble dyestuff-lemon yellow and sunset yellow in Adulterated Rhizoma Belamcandae.Method： Use TLC for iddentification and HPLC-DAD for iddentification and assay lemon yellow and sunset yellow.Result： Both TLC and HPLC-DAD can identify lemon yellow and sunset yellow in Adulterated Rhizoma Belamcandae,and HPLC detection indicate the content of lemon yellow between 0.11-0.37 g·kg-1,and sunset yellow between 0.04-0.11 g·kg-1.Conclusion： Staining Iridis kectori khizoma or other adulterants with lemon yellow and sunset yellow as Rhizoma Belamcandae for sales is not only unnecessary,but also increase unsafe factors in clinic.It is necessary to pay attention to about this by relative medicine researcher and manufactory.