降压中药有效组分配伍对离体大鼠胸主动脉环的舒张作用

Vasodilative Action of Combinations of Hypotensive Components of Traditional Chinese Medicines on Rat Isolated Thoracic Aorta

目的:研究天麻素、黄芩苷、川芎嗪、葛根素4种中药有效单体及其组合对大鼠胸主动脉的舒张作用,筛选出最佳组合。方法:以大鼠胸主动脉环为标本,观察单组分及组合物对去甲肾上腺素(NE)预收缩的血管舒张作用。结果:天麻素、黄芩苷、川芎嗪、葛根素单体存在浓度依赖性的舒张血管效应。正交实验结果显示天麻素(0.64 g.L-1)、黄芩苷(0.12 g.L-1)、川芎嗪(0.32 g.L-1)、葛根素(0.64 g.L-1)是最优组合,舒张率达到89.37%。结论:单组分及组合物对NE预收缩的大鼠胸主动脉均具有舒张作用,组合配伍强于单组分。

Objective：To investigate the vasodilative action of gastrodin,baicalin,tetramethylpyrazine,puerarin and their combinations on rat thoracic aorta,and to select the best combination of hypotensive components.Method： The rat thoracic aorta was taken as specimen and the vasodilative action of single components and their combinations on rat thoracic aorta which was pre-constricted with NE was tested.Result： Gastrodin,baicalin,tetramethylpyrazine and puerarin exerted a dose-dependent vasodilative action on rat isolated thoracic aorta rings.The results of orthogonal optimization experiment showed that the best combination including gastrodin（0.64 g·L-1）,baicalin（0.12 g·L-1）,tetramethylpyrazine（0.32 g·L-1） and puerarin（0.64 g·L-1） presented more obvious contraction than any single component.And the vasodilatation rate was 89.37%.Conclusion： The above components and their combination have vasodilative action on the NE pre-constricted rat thoracic aorta,but the effect of latter is stronger than the former.