四君子汤对肺癌恶病质小鼠骨骼肌萎缩及部分炎症细胞因子的影响

Effect of Sijunzi Decoction on Skeletal Muscle Atrophy and Some Inflammatory Cytokines in Lung Cancer Cachexia Mice

目的:观察四君子汤对肺癌恶病质小鼠骨骼肌萎缩的影响,并从炎性细胞因子的角度对其部分作用机制进行探讨。方法:利用lewis肺癌细胞株皮下接种C57B1/6小鼠,建立肺癌恶病质小鼠模型。将60只小鼠随机分为6组,荷瘤中药长期大剂量组,荷瘤中药短期大剂量组,荷瘤中药长期小剂量组,荷瘤中药短期小剂量组,荷瘤空白组,生理盐水对照组。监测各组小鼠的去瘤体重和腓肠肌重量,测量血清TNF-α和IL-6水平。结果:肺癌恶病质小鼠腓肠肌重量明显下降(P<0.05),血清TNF-α和IL-6水平明显升高(P<0.05),治疗组可以不同程度地缓解恶病质小鼠腓肠肌重量的下降,其中以长期大剂量组效果最优(P<0.05)。结论:四君子汤能够减缓肺癌恶病质小鼠骨骼肌萎缩的发生程度,其作用机制可能与减少部分炎性细胞因子的表达有关。

Objective：To observe the effect of Sijunzi Decoction on skeletal muscle atrophy in lung cancer cachexia mice,and to discuss the mechanism from inflammatory cytokines.Methods：To establish mice model of cancer cachexia by inoculating lewis lung cancer cell lines to C57B1/6 mice subcutaneously.60 mice were randomly divided into 6 groups.The tumor weight and gastrocnemius muscle weight were monitored,and the serum tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6）levels were measured.Results：The weight of gastrocnemius muscle were significantly decreased in the mice（P〈0.05）,serum TNF-α and IL-6 levels were significantly increased（P〈0.05）.In treated groups,the weight reduction of gastrocnemius in cachexia mice and the serum levels reduction of TNF-α and IL-6 were slight.Conclusion：Sijunzi Decoction could slowdown the cancer cachexia of skeletal muscle atrophy,and its mechanism may be associated with the decreasing expression of inflammatory cytokines.