HPLC测定清热消炎丸中黄芩苷的含量

Determination of Baicalin in Qingre Xiaoyan Pills by HPLC

目的:利用反相高效液相色谱法测定清热消炎丸中黄芩苷含量。方法:采用高效液相色谱(HPLC)C18柱,流动相为甲醇-0.1%磷酸水(47∶53);检测波长:280nm;柱温:25℃;流速:1mL/min;进样量:10μL。结果:黄芩苷在0.01648～0.0824mg/mL范围内线性关系良好,r=1,RSD=0.89%。结论:该方法操作简便,结果准确,重现性好,可用于清热消炎丸的质量控制。

Objective：To establish a quality RP-HPLC method for content determination of Baicalin in Qingre Xiaoyan Pills.Methods：The scopoletin was performed on a column C18;with a mobile phase of Methanol-0.1%H3PO4（47∶ 53）;the detective wavelength was 280nm;the column temperature：25℃;the flow rate was 1mL·min-1.Results：The content of Baicalin showed a good linearity in range of 0.01648-0.0824mg/mL（r=1.0,RSO=0.89%）.The average recoveryrate was 99.95% and RSD was 0.89%.Conclusion：The established qualitative and quantitative methods are simple,accurate,reliable and reproducible,and can be effectively used for the quality control of Qingre Xiaoyan Pills.