光交联透明质酸水凝胶的制备及性能

Preparation and Properties of Photocrosslinking Hyaluronan Hydrogel

采用甲基丙烯酸缩水甘油酯(GMA)对透明质酸(HA)进行化学改性,然后在紫外辐射下交联成水凝胶。随着GMA与HA物质的量比的增加(分别为10、20和50),HA分子链上的GMA取代度分别为2.8%、16.9%和45.2%,由此制得的水凝胶其交联点间平均分子质量分别为9.33×105g/mol、7.95×105g/mol、6.54×105g/mol;水凝胶的内部孔径逐渐变小;水凝胶的存储模量(G′)分别为70 Pa、101 Pa和160Pa,损耗模量(G″)分别为6.6 Pa、7.8 Pa和12.6 Pa;水凝胶的降解失重速率逐渐减慢,在120h时的失重百分率分别为63%、39.4%和30.8%。结果表明,GMA与HA物质的量比的增加有利于水凝胶交联密度的提高,力学性能的增强和降解速率的降低。

Various hyaluronan（HA） hydrogels were prepared under ultraviolet irradiation from glycidyl methacrylated（GMA） hyaluronan.With increasing the molar ratio of GMA and HA monomers,i.e.10,20 and 50,the degree of methacrylation is calculated to be 2.8%,16.9% and 45.2% respectively by 1H-NMR.For the obtained hydrogels,the average molecular weight between two cross-linked points is measured to be 9.33×105 g/mol,7.95×105 g/mol and 6.54×105 g/mol respectively.The pore size decreases with increasing GMA content by SEM.The rheological test shows the storage modulus of 70 Pa,101 Pa and 160 Pa respectively,and the loss modulus of 6.6 Pa,7.8 Pa and 12.6 Pa respectively.The mass loss rates reduce to 63%,39.4% and 30.8% respectively after degradation for 120 hours.These results indicate that increasing GMA content facilitates the increase of cross-linked density,the improvement of mechanical properties and the decrease of degradation rate.