耐氨基糖苷类铜绿假单胞菌相关耐药基因的研究

Researches on the correlated resistant genes of aminoglycosides-resistant Pseudomonas aerugionsa

目的了解广州地区耐氨基糖苷类抗生素铜绿假单胞菌的氨基糖苷类修饰酶(AMEs)基因和甲酰化酶基因的存在情况。方法用聚合酶链反应(PCR)测定临床分离的196株氨基糖苷类抗生素耐药的铜绿假单胞菌7种主要的AMEs基因和2种甲酰化酶基因。结果广州地区铜绿假单胞菌的AMEs基因携带率较高(93.37%),其修饰酶基因检出率分别为ant(3")-I(54.59%),aac(3)-II(44.89%),aac(6')-II(43.37%),aac(6')-I(36.73%),ant(2")-I(32.14%),aph(3')-IV(19.90%),aac(3)-I(0);同时携带2种或2种以上AMEs基因的菌株占70.92%;未检测到甲酰化酶基因阳性的菌株。结论广州地区铜绿假单胞菌中AMEs基因携带率较高,已出现多型别流行趋势,同一菌株携带多个不同的AMEs基因现象较多;尚未发现有甲酰化酶的流行。

Objective To investigate the existing situation of aminoglycoside modifying enzyme （AMEs） gene and methylation gene of aminoglycoside-resitant Pseudomonas aeruginosa in Guangzhou area. Methods 196 strains of aminoglycoside-resitant P. aeruginosa isolated in Guangzhou were detected f0r 7 main kinds of AMEs gene and 2 kinds of methylation genes by polymerase chain reaction（PCR）. Results Of the aminoglycoside -resitant P aeruginosa isolated in Guangzhou, 93.37% isolates were positive with the primers specific for AMEs. The detection rates of these genotypes were： ant（3＂）-I （54.59%）, aac（3）-II （44.89%）, aac（6＇）-II （43.37%）, aac（6＇）-I （36.73%）, ant（2＂）-I （32.14%）, aph（3＇）-IV （19.90%）, aac（3）-I （0）. The isolates rate that simultaneously carrying two or more genetypes was 70.92%, and the methylation genes were not detected in any of file aminoglycoside-resitant P aeruginosa isolated. Conclusion The P. aeruginosa isolated in Guangzhou that cadging the AMEs has a high level, and the genetype is multiple. Besides, there is no prevalence with methylation in Guangzhou at present. The phenomenon that carrying multiple genetypes in one isolates is quite a lot.