摘要
研究β-环糊精及其衍生物对AFG1的超分子包合作用及反应体系荧光响应值的影响。经研究:AFG1-HE-β-CD二元包合物反应体系荧光响应值大幅提高,在溶剂为2%的甲醇溶液,HE-β-CD浓度0.05mol/L、反应时间3min时体系可以达到最大的荧光值。根据Benesi-Hildebrand法确定HE-β-CD与AFG1形成1:1包络物。紫外吸收光谱、荧光光谱、KI淬灭实验以及热力学参数表明,AFG1进入HE-β-CD空腔从而荧光得到保护。AFG1在2～40μg/kg范围内与体系荧光呈良好线性关系,相关系数为0.9998,检出限为0.079μg/kg。将HE-β-CD应用于花生样品AFG1分析,准确度和精密度良好。
The fluorescence response signals of supramolecular inclusion complexes of AFG1 with β-cyclodextrin or its derivatives were studied. The fluorescence response signal of AFG1-HE-β -CD reaction system was significantly enhanced compared with AUG1, which reached maximum level after 3 min reation in 2% methanol solution HE-β3 -CD at the HE-β -CD cocnentration of 0.05 mol/L. According to the Benesi-Hidebrand equation, an inclusion complex was formed from AFG1 and HE-β -CD at a ratio of 1：1. UV spectroscopic, infrared spectroscopic and thermodynamic analyses and KI quenching experiments showed that AFG1 entered the cavity of HE-β-CD so that its fluorescence intensity was protected. An excellent linear relationship with fluorescence intensity was observed in the AFG1 concentration of 2 - 40μ g/kg （r = 0.9998）. The detection limit was 0.079μ g/L. Therefore, the application of HE-β -CD can permit the accurate and precise analysis of AFG1 in peanut samples.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第12期143-148,共6页
Food Science
基金
国家"863"计划项目(2007AA10Z427)
中央高校基本科研业务费专项(XDJK 2009C056)
西南大学研究生科技创新基金项目(ky2010006)
