固相微萃取与气相色谱-质谱联用分析信阳毛尖香气成分

Analysis of Aroma Components of Xinyang Maojian Tea by SPME-GC-MS

采用单因素对比试验方法确定固相微萃取信阳毛尖茶挥发性物质的优化条件,并采用气相色谱-质谱联用分析方法鉴定信阳毛尖香气组分。结果表明:固相微萃取与气相色谱-质谱联用分析信阳毛尖香气成分的最佳条件为萃取时间50min、萃取温度70℃,共鉴定出99种香气成分,其中醇类32种(相对含量47.83%)、碳氢化合物20种(相对含量10.97%)、酮类15种(相对含量20.43%)、酯类11种(相对含量7.24%)、酚类8种(相对含量3.4%)、醛类7种(相对含量7.73%)、杂氧化合物3种(相对含量1.01%)、含氮化合物3种(相对含量1.39%);信阳毛尖茶特征香气成分主要有芳樟醇、橙花醇、6-甲基-5-庚烯-2-酮、戊醇、β-紫罗酮、甲苯、5,6-环氧基紫罗酮、苯乙醇、Z-茉莉酮、庚醛、2,2,6-三甲基环己酮、苯甲醇、2-乙基己醇、橙花叔醇等。

Solid-phase microextraction （SPME） time and temperature were investigated for the analysis of aroma components in Xinyang Maojian tea by GC-MS. The optimal SPME extraction time and temperature were 50 min and 70 ℃, respectively. A total of 99 aroma components were identified including 32 alcohols （47.83%）, 20 hydrocarbons （10.97%）, 15 ketones （20.43%）, 11 esters （7.24%）, 8 phenols （3.4%）, 7 aldehydes （7.73%）, 3 mixed oxide compounds （1.01%）, and 3 nitrogen- containing compounds （1.39%）. The characteristic aroma components of Xinyang maojian were linalool, nerol, 6-methyl-5- heptene-2-ketone, amyl alcohol, β-ionone, toluene, 5,6-epoxy- β-ionone, phenethyl alcohol, Z-jasmone, heptaldehyde, 2,2,6 - trimethyl cyclohexanone, benzyl alcohol, 2-ethylhexyl alcohol, nerolidol, and so on.