摘要
目的分离和鉴定IRM-2小鼠辐射抗性相关基因。方法以IRM-2小鼠的21条表达序列标签为模板进行PCR扩增，从IRM-2小鼠全长cDNA文库中测定cDNA克隆的序列，通过与GenBank进行同源序列信息比对，对全长cDNA编码的蛋白质进行分析。结果从IRM-2小鼠全长cDNA文库中获得5条全长cDNA序列，与小鼠已知基因不同源，得到了可能编码蛋白质的氨基酸序列和蛋白质的理化性质等信息。结论通过对全长cDNA编码的蛋白质进行分析，提示IRM-2小鼠体内可能存在目前尚未发现的辐射抗性相关基因。
Objective To screen and isolate radioresistance-related genes from IRM-2 mouse. Methods Full-length cDNA products were amplified by PCR from IRM-2 mouse cDNA library according to twenty-one pieces of expressed sequence tags. The property of proteins encoded by full-length cDNA were analyzed by comparing with GenBank database. Results Five pieces of full-length cDNA which were not the same source as the known mice genes were found out from IRM-2 mouse cDNA library. Amino acid sequence and property of proteins encoded by these five pieces of full-length cDNA were obtained. Conclusion Proteins encoded by full-length cDNA imply that unkown radioresistance-related genes may exist in IRM-2 mouse.
出处
《国际放射医学核医学杂志》
2012年第3期157-159,共3页
International Journal of Radiation Medicine and Nuclear Medicine
基金
国家自然科学基金（30870583）
中国医学科学院放射医学研究所学科发展基金（SF0822）
