泽泻水煮液预防和治疗大鼠醉酒及保肝作用的实验研究

Experimental Study on Protective and Preventive Effects of Decoction of Rhizoma Alismasti in Acute Alcohol Poisoning Rats

目的:观察泽泻水煮液预防和治疗大鼠醉酒的作用及其机制。方法:64只Wistar大鼠随机分为空白组、模型组、预防大、中、小剂量组和治疗大、中、小剂量组。采用白酒灌胃法造模,每天灌胃后观察大鼠的行为学改变,每天实验前称大鼠体重,第7天用生物化学法检测血浆中的乙醇含量和肝匀浆中谷丙转氨酶(ALT)、谷草转氨酶(AST)、超氧化物歧化酶(Superoxide Dismutase,EC1.15.1.1,SOD)、丙二醛(malonyldialdehyde,MDA)的水平。结果:模型组大鼠血浆中乙醇含量明显升高(P<0.05),肝匀浆中ALT、AST水平明显升高(P<0.05),预防和治疗各剂量组可降低醉酒大鼠血浆乙醇含量和肝匀浆中ALT、AST水平。结论:泽泻水煮液可促进醉酒大鼠血浆中乙醇代谢,预防酒精中毒,从而达到保护肝脏功能的目的。

Objective：To investigate the effects of decoction of rhizoma alismasti on preventing and treating alcoholism,and explore its mechanism.Methods：64 healthy Wistar rats were divided into control group,poisoning group,three prevention groups（large,middle,small）,three treatment groups（large,middle,small）.The blood and liver tissue of each rat were obtained to test the levels of the blood concentration of ethanol,ALT,AST,SOD and MDA.Results：The levels of the blood concentration of ethanol,ALT and AST were significantly increased in poisoning group as compared with those in control group.Conclusions：The levels of the blood concentration of ethanol,ALT and AST were increased in acute alcohol poisoning rats.Rhizoma alismasti plays a protective role on acute alcohol poisoning by promoting the ethanol metabolism,so the damage of liver was decreased.