低氧大鼠肺、肠组织VIP、P物质及CCK的表达差异分析

Analysis on the Contents of VIP,SP and CCK in Lung and Intestine in Rat Model of Low-O2

目的:分析不同状态下肺与空肠、回肠、结肠组织上的VIP、P物质、CCK表达差异,探讨肺与大肠的表里关系及它们之间的基础物质。方法:20只SD雌性大鼠随机分为正常组,低氧组,每组10只。低氧组大鼠给予每天8h低氧刺激,7天后取材。用放射免疫的方法测定两组大鼠肺、空肠、回肠、结肠组织的VIP、CCK及P物质的含量。结果:正常组大鼠的肺VIP、P物质及CCK与空肠组织VIP、P物质及CCK相比有明显差异(P<0.05),与回肠、结肠没有明显差异(P>0.05);低氧组大鼠的肺VIP、P物质及CCK与空肠相比也具有明显差异(P<0.05),与回肠、结肠组织相比没有明显差异(P>0.05)。结论:正常组大鼠肺与回肠、结肠没有差异,低氧组大鼠肺与回肠、结肠也没有差异。肺与大肠具有相表里关系。VIP、P物质及CCK是肺肠之间功能联系的物质基础。

Objective：To observe the contents of VIP,SP and CCK in lung and intestine in different states,analysis on the relationship and the base material between the lung and intestine was made.Methods：The rat model of low-O2 was established,8hs everyday,and then the rats were killed after 7 days.VIP,SP and CCK were measured with Radioimmunoassay.Result：There were no significant differences in the contents of VIP,SP and CCK in lung and intestine in different states.Conclusion：The lung and the large intestine are interior-exteriorly related.VIP,SP and CCK are the base material between the lung and intestine.