正交试验优化微波辅助乙醇回流提取洋葱黄酮的工艺研究

Study on the Technology Optimization of Extract Flavones from Onion by Ethanol Refluxing Associated with Microwave Pretreatment

目的:以黄酮含量为指标,探讨微波预处理提取洋葱中黄酮的较佳提取工艺。方法:用紫外分光光度法建立洋葱黄酮含量的测定方法,以不同微波预处理时间,乙醇浓度,微波功率,料液之比为考察因素,用正交试验法考察洋葱黄酮的较佳提取工艺。结果:较佳提取条件为:在微波功率900W,微波时间5 min,70%乙醇,料液之比为1∶10条件下,黄酮的提取率最高。结论:采用洋葱黄酮微波-乙醇提取法提取洋葱中黄酮是可行的,且相对其他提取黄酮的方法,具有高效、易操作的特点,本实验为优化洋葱黄酮的提取工艺奠定了基础,并可为含洋葱黄酮的相关制剂的含量测定提供方法。

Objective：The technological process of extracting flavones from onion using microwave pretreatment-ethanol refluxing method was studied in this paper.Methods：Extraction technology of flavones in onion was studied by UV spectrophotometry and orthogonal experiment.Effects of microwave pretreatment time,ethanol-water solvent,microwave power,ratio of material to liquid on extraction rate of flavones were investigated.Results：The optimum extraction conditions for onion flavones were established as follows：microwave power 900W,microwave time：5min,80% ethanol,the ratio of material to liquid was 1∶ 10.Conclusion：The method of study on microwave-alcohol process in onion flavones is feasible and has high efficiency,which is easy to be operated compared to other flavones extraction methods.The experiment laid a foundation of optimizing the extraction process of flavones in onion and can provide methods for the determination of preparations associated with onion flavones.