蛛丝对家兔颈动脉的亚急性阻断作用

Subacute Blocking Effect of Spider Silk on Rabbit Carotid Artery

目的:尝试以蛛丝为新材料探索建立亚急性颈动脉缺血模型,并评论此模型对相关生理生化指标的影响,从而为相关疾病的研究提供新的方法。方法:先从蜘蛛中绕取长约10cm的蛛丝备用。将家兔颈动脉分离好后,再将准备好的蛛丝缠绕于颈动脉上,形成结扎点,建立亚急性缺血模型,并测定血流、血压、SOD等各项指标。结果:蛛丝结扎后的颈动脉血流、血压总体表现出缓慢下降的趋势。血清MDA,SOD在结扎后首先明显升高,随后又缓慢下降,大体病理观察发现蛛丝对颈动脉的作用过程是一个从部分阻断到完全阻断的渐进过程。结论:利用蛛丝结扎可以建立血管的亚急性缺血模型,将来可能将其应用到人类慢性缺血性疾病的模型中去。

Objective：Try to explore subacute carotid ischemia model established by spider silk,and comment on the influence on relevant physiological and biochemical indices,thus provide a new method for related disease research.Methods：First take about 10cm of silk from spider.The carotid separation of rabbit was made,then prepared spider silk wound in carotid artery to make ligation points,so as to establish subacute ischemia model.And the determination of blood flow,blood pressure and SOD were observed.Results：After spider silk ligation carotid,the blood flow and blood pressure had a decrease trend.Serum levels of MDA and SOD were increased significantly after ligation,then slowly declined.General pathology observation on carotid process is from partially block to completely block.Conclusions：Using spider silk ligation can build vascular subacute ischemia model,which may be applied to models of chronic ischemic diseases in the future.