HPLC测定五味麝香丸中乌头类生物碱的含量

Simultaneous Determining on the Content of Three Aconitine-Type Alkaloids in Wuwei Shenxiang Pills by HPLC

目的:建立高效液相色谱同步测定五味麝香丸中主要毒性成分乌头碱、次乌头碱和新乌头碱的含量。方法:色谱柱:Thermo Hypersil GOLD C18(250 mm×4.6 mm,5μm);流动相:甲醇-水-氯仿-三乙胺(100∶50∶3∶0.10);流速:1 mL·min-1;检测波长:235 nm;柱温:30℃。结果:乌头碱在3.28～82.0μg·mL-1(r=0.9998)、次乌头碱在3.2～80.0μg·mL-1(r=0.9998)和新乌头碱在3.2～80.0μg·mL-1(r=0.9998)浓度范围内呈良好的线性关系。乌头碱、次乌头碱和新乌头碱回收率分别为99.2%(RSD=0.58%)、101.1%(RSD=0.89%)和99.6%(RSD=0.35%)。结果本实验建立的方法简便可靠、专属灵敏,可用于五味麝香丸的质量控制。

Objective：To establish the quantitative method of simultaneous determining the content of aconitine,hypaconitine and mesaconitin,three main toxic components in Wuwei Shenxiang Pills.Methods：Column：Thermo Hypersil GOLD C18（250mm×4.6mm,5μm）;mobile phase：methanol-water-chloroform-triethylamine（100∶ 50∶ 3∶ 0.10）;flow rate：1.0mL·min-1：detection wavelength：235 nm;column temperature：at 30 ℃.Results：Aconitine,hypaconitine and Mesaconitin showed a good linear relationship at the range of 3.28～82.0μg·mL^-1（r=0.9998）,3.2～80.0μg·mL^-1（r=0.9998）and 3.2～80.0μg·mL^-1（r=0.9998）.The recovery of aconitine,hypaconitine and mesaconitin was 99.2%（RSD=0.58%）,101.1%（RSD=0.89%）and 99.6%（RSD=0.35%）.Conclusion：This method is simple,easy,specific and applicable,and can be used for quality control in Wuwei Shenxiang Pills.