氧化苦参碱干预人体骨肉瘤细胞增殖周期 诱导其凋亡的作用

目的研究氧化苦参碱对人体骨肉瘤细胞（MG-63）凋亡的影响。方法在培养液体中加入不同浓度的氧化苦参碱（Oxymatrine，OM）进行不同时间长度的诱导，以流式细胞术法检测人体骨肉瘤细胞（MG-63）增殖、周期及凋亡情况。结果1～50μmol／L氧化苦参碱均能抑制骨肉瘤细胞增殖，同时能够阻滞MG-63细胞进入S和G2／M期，使细胞停滞于G0／G1期，与空白对照组比较，差异有统计学意义，且氧化苦参碱高剂量组最明显（P〈0．05）。5、10、50μmol／L氧化苦参碱作用细胞96h后的凋亡率分别为（10．96±0．41）％、（20．74±1．09）％、（27．05±2．00）％，与对照组（4．66±1．26）％比较，差异均有统计学意义（均P〈0．05）。氧化苦参碱浓度越高，抑制作用越明显，呈明显的剂量依赖效应。50¨mol／L氧化苦参碱作用于细胞48h、72h、96h后的凋亡率分别为（6．65±1．49）％、（14．82±5．79）％、（27．05±2．00）％，与对照组各时间点（4．01±0．86）％、（4．31±0．68）％、（4．66±1．26）％比较，差异均有统计学意义（均P〈0．05）。氧化苦参碱作用时间越长，抑制作用也越明显，呈明显的时间依赖效应。结论氧化苦参碱可通过时间依赖性和剂量依赖性方式，促使细胞G2／M期阻滞和抑制细胞有丝分裂，从而诱导人体骨肉瘤细胞凋亡。

Objective To study the apoptosis and proliferation of osteosarcoma cells （ MG-63 ） with the treatment of oxymatrine （OM） . Method OM with different concentrations was added in culture medium system for various lengths of time of induction, was detected by flow cytometry in human osteosarcoma cells （ MG-63 ） cell proliferation, cell cycle and apoptosis. Results 1-50 pμmol/L matrine could inhibit oxidation of osteosarcoma cells, and block MG-63 cells, cells from entering into the S and G2/M phase, the cells were arrested in G0/G1 phase. Compared with the blank control group, there was a significant difference, with the high dose group most significant, the most significant （ P〈0.05 ） .It was also significantly different in oxymatrine plus cisplatin group and blank control group （P〈0.05） . The apoptosis rates of 5, 10, 50 μmol/L oxymatrine in cell for 96h were （ 10.96 ± 0.41 ） %, （ 20.74 ± 1.09 ） %, （ 27.05 ± 2.00 ） %, and difference was significant compared with that in the control group [ （ 2.07 ± 1.06 ） % ] （ all P〈0.05 ） .Oxymatrine higher the concentration of the more obvious inhibition, showing a clear dose-dependent effect. The apoptotic rates with 50 p~ mol/L oxymatrine in cell for 48h, 72h, 96h were （ 6.65 ± 1.49 ） %, （14.82 ±5.79）%, （27.05±2.00）%, With the control group at all time points [ （4.31±0.68）%, （4.01±0.86）%, （ 4.66 ± 1.26 ） % ] , the differences were significant （ P〈0.05 ） .Oxymatrine were more effective with longer time of inhabitatation, with an obvious time-dependent effect. Conclusion Oxymatrine can promote cell G2/M phase arrest and inhibit cell mitosis, thereby inducing apoptosis in human osteosarcoma cells with time-dependent and dose-dependent manner.