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杜仲对单侧输尿管梗阻大鼠肾间质纤维化的影响 被引量:13

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摘要 目的观察杜仲对单侧输尿管梗阻(UUO)大鼠肾间质纤维化的影响并探讨其可能机制。方法采用UUO肾间质纤维化模型。将32只大鼠随机分为4组,每组8只,即假手术组、UUO组、厄贝沙坦组和杜仲组。厄贝沙坦组给予厄贝沙坦10 mg/(kg.d)、杜仲组给予杜仲6 g/(kg.d)治疗。术后2周处死大鼠,光镜下观察Masson和HE染色肾间质纤维化指数,观察肾组织病理改变,并应用免疫组织化学方法检测肾组织结缔组织生长因子(CTGF)和基质金属蛋白酶-2(MMP-2)的表达。结果①UUO组与假手术组相比,BUN、Scr水平均显著增加;杜仲组较UUO组呈下降趋势;厄贝沙坦组作用效果与杜仲组相似(P>0.05)。②与假手术组相比,肾组织HE染色UUO组可见弥漫的肾小管上皮细胞空泡变性、扩张、部分萎缩,偶见坏死、肾间质增宽、大量炎细胞浸润和间质纤维化,肾小球病理改变不明显;杜仲组上述改变均有所减轻;厄贝沙坦组病理改变与杜仲组相似(P>0.05)。③与假手术组相比,Masson染色结果显示UUO组肾间质增宽、大量胶原纤维增生、肾小球病变不明显;杜仲组上述改变均有所减轻;厄贝沙坦组病理改变与杜仲组相似(P>0.05)。④假手术组大鼠肾间质有少量CTGF表达,染色为颗粒状,着色稀疏;UUO组CTGF表达明显增多,呈不均匀棕黄色颗粒状,肾小球表达不明显;杜仲组CTGF表达较UUO组明显降低;厄贝沙坦组CTGF表达与杜仲组相似(P>0.05)。⑤MMP-2在假手术组大鼠肾间质中呈棕黄色颗粒状表达;UUO组中大鼠肾间质仅有少量表达且着色稀疏;杜仲组MMP-2表达较假手术组减轻,但其表达仍高于UUO组;厄贝沙坦组与杜仲组相似(P>0.05)。结论杜仲可抑制CTGF的过度表达和增强MMP-2的表达,进而减缓肾纤维化的病程进展。
出处 《山东医药》 CAS 2012年第28期47-49,F0003,共4页 Shandong Medical Journal
基金 湖北省卫生厅资助项目[鄂卫发(2006)63号-19]
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