FSHR基因第10外显子多态性及其与小梅山猪产仔数的相关性

Polymorphism of Exon10 of FSHR Gene and Its Relationship with Litter Size in Xiaomeishan Pigs

【目的】研究猪FSHR基因第10外显子的多态性及其与小梅山母猪产仔数的相关性。【方法】采用PCR-SSCP和直接测序方法检测基因多态性,利用最小二乘法分析其对母猪产仔数的关系,同时对多态位点的方差组分进行分析,并预测选择反应。【结果】在小梅山猪、枫泾猪和大白猪这3个群体中,引物P1和P3上分别检测到1个多态位点,其中P1位点可导致氨基酸的改变;引物P6仅在大白猪中检测到1个多态位点;对小梅山猪而言,P1位点的A等位基因和P3位点的D等位基因为优势基因;在P1位点上,两胎以上小梅山母猪中,AA型个体的总产仔数(total number born,TNB)和产活仔数(number born alive,NBA)比BB型分别高出1.95和1.66头(P<0.01);在P3位点上,在两胎以上及所有胎次的母猪中,DD型个体的TNB和NBA均极显著高于CC型(P<0.01);小梅山母猪的TNB和NBA在P1和P3位点上的遗传主要受到加性效应的影响。【结论】FSHR基因第10外显子P1和P3位点的A和D等位基因对小梅山猪产仔数有显著影响。

[ Objective ] The effect of polymorphism of exonl 0 of F SHR gene on litter size in Xiaomeishan pigs was studied. [Method] The polymorphism of exonl0 of FSHR gene was investigated by PCR-SSCP and direct sequencing methods, and the effect of exonl 0 of FSHR gene on litter size traits in Xiaomeishan pigs was analyzed using the least square analysis. Simultaneously the variance components were analyzed and selective reaction was predicted. [Result] There was one novel polymorphic locus dected by primers P1 and P3, respectively, and the former made amino acid change, among Xiaomeishan, Fengjing and Large White pigs. One polymorphic locus detected by primer P6 was only in Large White pigs. Allele A at locus P1 and allele D at P3 locus were the dominant alleles in Xiaomeishan pigs. AA genotypic sow of Xiaomeishan pigs produced 1.95 TNB （total number born） and 1.66 NBA （number born alive） more than BB genotypic sow at locus P1 after the second parity, respectively （P〈0.01）. And DD genotypic sow of Xiaomeishan pigs produced more TNB and NBA than CC genotypic sow at locus P3 after the second parity and in all parities, respectively （P〈0.01）. The heredity of TNB and NBA in Xiaomeishan pigs was mostly influenced by the genetic additive effect. [Conclusion] Allele A at locus P1 and allele D at locus P3 in exonl0 ofFSHR gene had significant effect on litter sizes in Xiaomeishan pigs.