促孕散对不孕奶牛血清中微量元素和白介素的影响

Effects of Fertility-Promoting Powder on Trace Elements and Interleukin in Serum in Infertile Dairy Cattle

【目的】探讨经促孕散对不孕奶牛治疗前后血清中微量元素(Cu、Fe、Mn、Zn)与白介素(IL-1、IL-6)的含量变化规律。【方法】利用火焰法、原子吸收光谱法和酶联免疫吸附法检测实验组和正常组治疗前、后血清中微量元素和白介素的含量。【结果】①促孕散治疗持久,黄体有效率为91.67%,卵巢静止有效率为88.89%,②持久黄体型治疗前血清中Cu、Zn含量显著高于正常组(P<0.05),Mn含量极显著低于正常组(P<0.01),治疗后血清中含量接近正常,且含量水平差异不显著(P>0.05),Fe、IL-1、IL-6含量治疗前后与正常组比较差异不显著,③卵巢静止型治疗前血清中Mn含量低于正常组(P<0.01),IL-1含量高于正常组(P<0.05),治疗后逐步与正常组含量水平差异不显著(P>0.05),Cu、Fe、Zn、IL-6含量治疗前后与正常组比较差异不显著(P>0.05)。【结论】促孕散对不孕奶牛血清中部分微量元素和白介素含量有微调作用,在奶牛不孕症治疗效果上有不可忽视的作用。

[Objective] The law of trace elements （Cu, Fe, Mn, Zn） and interleukin （IL-1, IL-6） contents in serum using fertility-promoting powder to treat infertility caused by ovarian disease of dairy cattle were studied. [Method] Flame atomic absorption spectrometry and enzyme-linked immunization absorb assay were used to detect the levels of trace elements and interleukin contents in serum from the experimental and normal groups before and after treatment. [Result] After treatment, the effective rate of persistent corpus luteum was 91.67%, the effective rate of ovarian static was 88.89%. In persistent corpus luteum body, Cu, Zn contents were significantly higher than normal group in pre-treatment （P〈0.05） , Mn content was significantly lower than the normal group （P〈0.01）, after treatment, their content levels closed to the normal group, and the difference was not significant （P〉 0.05）. The Fe, IL-1, and IL-6 contents closed to the normal group before and after treatment. In ovarian static body, Mn content was significantly lower than the normal group in pre-treatment （P〈0.01）, IL-1 content was higher than the normal group （P〈0.05）, after treatment, the contents were not significantly different （P〉0.05）, but Cu,Fe,Zn, and IL-6 contents were not significantly different compared with the normal group before and after treatment （P〉0.05）. [ Conclusion ] The effect of fertility-promoting powder on trace elements and interleukin in serum can not be ignored in treatment of infertility caused by ovarian disease of dairy cattle.