河西绒山羊次级毛囊超微结构的周期性变化

Identification of the Secondary Follicle Cycling of Hexi Cashmere Goat

【目的】研究成年绒山羊次级毛囊组织超微结构的周期性变化过程,为研究绒山羊绒毛生长的分子调控机理奠定形态学基础。【方法】活体采集的皮肤样品,经3%的戊二醛前固定和锇酸的后固定,通过上行酒精梯度脱水后进行包埋。制作河西绒山羊一年周期内每个月的皮肤超薄切片,经铀和铅双染色后在透射电镜下进行超微结构的观察、拍照和分析。【结果】河西绒山羊次级毛囊组织的超微结构随着毛生长周期呈周期性变化,可分为5个不同的时期,即1—2月为休止期、3—4月为生长前期、5—8月为生长期、9—10月为退行前期、11—12月为退行期。【结论】阐述了河西绒山羊次级毛囊在一个完整的生长循环过程中所处的5个不同的时期,为绒山羊绒毛相关领域的研究奠定超微结构方面的基础。

[ Objective ] An experiment was conducted to identify the periodic change of ultrastructures of secondary follicle characteristics in the whole year, reveal the molecule regulation of growth of cashmere goat. [ Method] A total of six cashmere goats of one year old were used in this experiment. The ultrathin slices of skin were made each month in the whole year, observed and photographed under transmission electron microscope after staining. [Result] Following the development of down fiber, the ultrastructures of secondary follicle of Hexi cashmere goat showed a periodic change within a year. The.re were five different periods during the down fiber cycle. It was observed that the stages oftelogen, pregrowing, anagen, precatager and catagen were in January and February, March and April, May to August, September and October and November and December, respectively. [Conclusion] This study illustrated the five different stages of secondary follicle of Hexi cashmere goat within a whole growing cycle, and has provided detailed information on the research field of cashmere goat.