大麻品种遗传多样性的AFLP分析

Genetic Diversity of Cannabis sativa L.Based on AFLP Analysis

利用POPGENE 3.2软件对13个不同来源的大麻群体进行遗传多样性分析。结果显示:云南地区的大麻群体具有最高的遗传多样性水平(PPB=88.82%,He=0.3000,I=0.4571),其次为黑龙江群体(PPB=75.66%,He=0.2572,I=0.3897)。13个大麻群体的多态位点百分率(PPB)为92.11%,Nei's总遗传多样性(Ht)为0.3837,Shannon's信息指数I=0.5374。群体内遗传多样性(Hs)为0.1640,群体间的遗传分化系数(Gst)为0.5725,总的遗传变异中有57.25%发生在群体间,42.75%发生在群体内。根据Nei's(1978)的方法计算了13个大麻群体间的遗传距离和遗传一致度。结果显示:各群体间的遗传一致度在0.6556～0.9258之间,其中四川群体和广西群体间具有最高的遗传一致度(0.9258);云南群体与贵州群体和四川群体间遗传一致度分别为0.9196、0.9173。所有群体中甘肃群体和山西群体遗传一致度最低为0.6556,说明大麻种内具有较大的遗传变异。

Genetic diversity of 13 Cannabis populations from different sources was analyzed by POPGENE 3.2 Software.AFLP analysis showed that the Yunnan population had the highest level of genetic diversity（PPB=88.82%,He = 0.3011,I= 0.4571）,and then the Heilongjiang population（PPB=75.66%,He=0.2572,I= 0.3897）.The percentage of polymorphic loci（PPB） of 13 Cannabis populations was 92.11%.Nei′s total genetic diversity（Ht） was 0.3837,the genetic diversity（Hs） was 0.1640.Coefficient of genetic differentiation among populations（Gst） was 0.5725,it means that 57.25% of the total genetic variation occurred among populations,and 42.75% genetic variation in different populations.To further analyze the genetic differentiation among populations,the genetic distance and genetic identity of Cannabis were calculated according to Nei′s（1978） method.The results showed that the genetic identity among populations was from 0.6556 to 0.9258,the highest degree of consensus between Guangxi population and Sichuan population was 0.9258.Genetic identity between Yunnan population and Guizhou population or Sichuan population were 0.9196 and 0.9173.Gansu and Shanxi population with lowest genetic identity in all populations was 0.6556.The results indicated that rich genetic diversity among 13 cannabis populations.This study analyzed genetic diversity of cannabis populations in molecular level,provided scientific evidence for the protection of seeds,breeding,evolution study of industrial hemp.