海岛棉(Gossypium barbadense L.)产量和早熟性状QTL定位

QTL Mapping of Yield and Earliness-related Traits in Sea-island Cotton(Gossypium barbadense L.)

对海岛棉产量和早熟性状进行QTL初步定位,为分子标记辅助育种提供依据。利用5200多对SSR引物筛选海岛棉品种新海3号和Giza82间的多态性引物,获得107对。以多态性引物检测新海3号×Giza82的190个F2:3家系,获得120个多态性位点。利用JoinMap3.0分析软件构建了一个包含22个连锁群,74个标记,标记间平均距离12.06 cM,全长893 cM,覆盖海岛棉基因组20.12%的分子标记遗传连锁图谱。采用复合区间作图法检测到21个与海岛棉产量性状和早熟性状有关的QTL,其中早熟性状检测到12个QTL,分别位于1、3、5、6、11、17、22共7个连锁群上;产量性状检测到9个QTL,分别位于1、4、5、6、7、16、22共7个连锁群上。研究结果为海岛棉产量性状和早熟性状的分子设计育种提供了有用的信息。

QTL mapping on the yield and earliness-related traits of the sea-island cotton can facilitate using DNA molecular markers in cotton improvement.More than 5200 pairs of SSR primer were used to screen the polymorphism between two sea-island cotton cultivars ’Xinhai 3’and ’Giza 82’,finally 107 pairs of polymorphic primer were obtained.Using SSR technique with the 107 pairs of polymorphic primer,190 F2∶ 3 family lines derived from the two sea-island cotton cultivars were analyzed,which produced 120 polymorphic loci.Using JoinMap3.0 software,a genetic linkage map of sea-island cotton with 74 loci was constructed,which comprised of 22 linkage groups covering 893 cM with an average interval of 12.06 cM between the two neighbor markers,and approximately 20.12% of the whole cotton genome.Based on composite interval mapping.21 QTL related with yield and earliness traits of the sea-island cotton were detected.12 QTL associated with earliness traits were respectively located on linkage groups LG1、 LG3、 LG5、 LG6、LG11、LG17、LG22 and 9 QTL associated with yield traits were respectively located on linkage groups LG1、LG4、LG5、LG6、LG7、LG16、LG22.This research could provide valuable information for the molecular design breeding of yield and earliness in sea-island cotton.