浙南柚类地方资源遗传多样性分析和鉴定

Genetic Diversity Analysis and Identification of Pummelo Germplasms in Southern Zhejiang Province

利用SRAP标记对13份浙南柚类地方资源和琯溪蜜柚及芽变进行遗传多样性分析和鉴定。结果表明:平均每个引物组合可扩增出15.7条谱带,14对SRAP引物共产生220条谱带,其中多态性谱带为122条,多态率为55.4%,表明15份材料间检测到的SRAP位点多态性不高,不同引物组合可将11个基因型完全分开。聚类分析结果显示,15份柚类种质在遗传相似系数0.97处可以分为8大类,第1类群为四季柚7个优异株系,第2类群包括琯溪蜜柚及其芽变,而平阳文旦、早香柚、处红柚、红心1号土柚、红心2号土柚、酸柚分别单独为第3、4、5、6、7、8类群。四季柚选系中务城1号、务城3号、马站红心四季柚的指纹图谱有可区分的差异,说明DNA水平发生轻微变异。

Sequence related amplified polymorphism（SRAP） markers were used to evaluate the genetic diversities of 13 pummelo（C.maxima） germplasms collected from southern Zhejiang province and Guanxi miyou pummelo and its bud mutant from Fujian province.Fourteen SRAP primer combinations were thus selected and a total of 220 PCR fragments with an average of 15.7 bands per primer combination were obtained.Among these bands,122（55.4%） were polymorphic,showing a relatively low level of genetic diversity among the analyzed germplasms.Eleven genotypes were uniquely identified in the 15 germplasms by SRAP markers.UPGMA Clustering analysis of the SRAP banding patterns showed that the 15 pummelos could be divided into 8 major groups at the similarity coefficient level of 0.97.The first group included the 7 Sijipao varieties.The second group included Guanxi miyou and its bud mutant hongrou miyou.Each of the rest pummelos,i.e.,Pingyang pummelo,Zaoxiang pummelo,Chuhong pummelo,Hongxin 1 pummelo,Hongxin2 pummelo and Sour pummelo,was clustered as a separate group.It showed that slight change occurred at DNA level in Wucheng1,Wucheng3,Mazhanhongxin pummelo based on genetic diversities.