黄瓜核心种质低温耐受性的田间评价

Field Evaluation of Resistances to Low Temperature in Core Collections of Cucumber Germplasm

在自然低温条件下,利用幼苗存活率、冷害指数、叶片数、株高及叶绿素含量5个指标对303份黄瓜核心种质进行了低温耐受性评价,结果表明:5个指标基本符合正态分布的特点,幼苗存活率和冷害指数均有明显向低值和高值区域偏离的趋势。相关分析结果显示冷害指数与幼苗存活率、叶片数、株高及叶绿素含量4个指标呈极显著负相关。通过隶属度函数综合评价群体的低温耐受性,共筛选获得28份耐低温种质,为黄瓜耐低温的品种选育及进一步深入挖掘低温耐受性相关基因奠定了基础。

Under the natural condition of cold stress,5 indexes including chilling injury index,seedlings surviving rate,number of leaves,plant height and chlorophyll content were chosen to evaluate the tolerance to low temperature of 303 cucumber core collections.Results showed that the five indexes generally accorded to a normal distribution while the seedling surviving rate and chilling rate significantly deviated to low value and high value area.Correlation analysis revealed a significantly negative correlation between the chilling index and the other four indexes including seedling surviving rate,number of leaves,plant height and chlorophyll content.Using membership function to comprehensively evaluate the tolerance to low temperature of core collections,28 different cucumber germplasm resources with high tolerance to low temperature were screened out,which set the foundation both for breeding and excavate elite genes related with tolerance to low temperature further in cucumber.