太湖稻区及国内部分香稻SSR指纹图谱构建及遗传多样性初析

Establishment of SSR Fingerprint Map and Preliminary Analysis of Genetic Diversity among Aromatic Rice from Taihu and other Areasin China

以太湖稻区和国内其他地区的39个香稻品种,以及籼型恢复系2个对照品种为研究材料,利用SSR分子标记进行DNA指纹图谱构建和遗传多样性分析。从40对国标中公布的水稻SSR引物中筛选出13对核心引物,在41个水稻品种中共检测到36个多态性片段。据此建立41个水稻品种的DNA指纹图谱,进行聚类分析,发现供试41个品种的遗传相似系数在0.58以上,而供试香稻品种的遗传相似系数在0.64以上,基本反映了不同品种间的亲缘关系。

41 rice lines,including 39 aromatic rice lines and 2 indica rice restorer lines were analyzed by using 13 simple sequence repeats（SSR）primer pairs selected from 40 SSR of National Standard primer pairs that disperse on 12 chromosomes in rice.A total of 36 polymorphic bands were detected among 41 lines.Cluster analysis showed that genetics similarities among the 41 lines were above 0.58,while the genetics similarities among the aromatic rice lines were above 0.64.As expected,the result of cluster reflected basically the genetic relatives based on the pedigree analysis.