紫杉醇对人肝癌细胞株MHCC97-H的细胞形态和周期的影响

Effects of paclitaxel on cellular morphous and cell cycle of human hepatoma carcinoma cell strain MHCC97-H

目的研究紫杉醇对体外培养肝癌细胞MHCC97-H的细胞形态和细胞周期的影响,初步探讨其作用机制。方法 (1)将MHCC97-H传代培养后,给予不同浓度的紫杉醇作用24 h,透射电镜观察细胞形态特征;(2)不同浓度的紫杉醇分别作用MHCC97-H 12、24、48 h后,MTT法检测肝癌细胞的抑制率,并绘制药物浓度-细胞抑制率曲线;(3)不同浓度的紫杉醇作用MHCC97-H 48 h后,用流式细胞仪测定细胞周期的变化及凋亡情况。结果 (1)随着紫杉醇浓度的提高,MHCC97-H的细胞伪足数量减少;(2)紫杉醇对体外培养的MHCC97-H生长具有明显抑制作用,并且存在剂量与时间依赖性;(3)紫杉醇可以将MHCC97-H阻滞于G2-M期,并促进其凋亡。结论紫杉醇可以将MHCC97-H大部分阻滞于G2-M期,并通过诱导肝癌细胞凋亡来协同发挥抗癌作用,同时使肝癌细胞伪足减少来降低其运动能力。紫杉醇可能是有潜力的抗肝癌生长及转移的药物。

Objective To investigate the effects of paclitaxel on the cellular morphous and cycle of hepatoma carcinoma cell MHCC97 - H which was cultured in vitro, and to discuss its mechanism of action. Methods （ 1 ） After the serial subeultivation of MHCC97 - H, different concentrations of paclitaxel were used to treat the cells for 24 h. Transmission electron microscope was used to observe the cellular morphologic characteristics. （2）After the treatment of MHCC97 - H with different concentrations of paclitaxel for 12,24, and 48 h, MTY assay was used to detect the inhibition ratio of hepatoma carcinoma cell, and the drug concentration - cellular inhibition rate curve was drawn. （ 3 ） After the treatment of MHCC97 - H by different concentrations of paclitaxel for 48 h ,flow cytometry was used to detect the changes of cell cycle and apoptosis. Results （ 1 ） Along with the increase of paclitaxel concentration, the pseudopodium amount of MHCC97 - H cell decreased. （2） Paelitaxel had significant inhibitive effect on the growth of MHCC97 - H in vitro,and there was a dose - time dependence. （3）Paelitaxel could arrest MHCC97 - H cell within the G2 - M phase and induce the apoptosis. Conclusion Paclitaxel can arrest most of the MHCC97 - H cells within the G2 - M phase. It has the effect of anti - tumor through reducing the apoptosis of hepatoma carcinoma cells and reduces the pseudopodium amount of carcinoma cell to decrease the moving ability of MHCC97 - H cell. Therefore,it is a potential drug of anti - growth and anti - metastasis of hepatic cancer.