紫檀芪对人食管癌EC9706细胞生长的影响及其机制

Research on effects of pterostilbene on growth of human esophageal cancer cell EC9706 and its mechanism

目的探讨紫檀芪(PTT)对人食管癌EC9706细胞生长影响及其抗凋亡的机制。方法 EC9706细胞分为对照组(正常DMEM培养液孵育)和PTT组(DMEM培养液中含PTT,分别为15、30、45、60μM),各组细胞分别处理24、48、72 h。MTT法检测各组细胞存活率;黏附实验测定各组细胞黏附率;Western blot方法检测各组细胞抗凋亡相关蛋白Bcl-2和凋亡相关蛋白Caspase-3表达。结果 MTT结果显示,PTT对EC9706细胞生长有抑制作用,其中60μM处理72 h组抑制效果最明显,呈药物浓度及时间依赖效应;黏附实验结果显示,PTT处理72 h可以显著减弱EC9706细胞黏附能力,并呈浓度依赖效应;Western blot结果显示(72 h):PTT处理72 h可以显著降低EC9706细胞Bcl-2的表达,同时显著升高其Caspase-3的表达。结论紫檀芪可抑制EC9706细胞生长,促进其凋亡,其机制与激活Caspase-3并抑制Bcl-2表达有关。紫檀芪可能是食管癌治疗领域具有开发前景的新药。

Objective To investigate the effects of pterostilbene（FTT） on the growth of human esophageal cancer cell EC9706 and its anti - apeptosis mechanism. Methods The EC9706 ceils were divided into control group （receiving incubation in normal DMEM culture fluid）and PTr groups（receiving incubation in the DMEM culture fluid containing 15,30,45,and 60 p,M PTT） ,and those cells in each group were treated respectively for 24,48 ,and 72 h. MTT assay was used to detect the survival rate of those cells. Adhesion assay was carried out to determinate the adhesive rate of those cells. Western blot was used to detect the expression of anti - apoptotic protein Bcl - 2 and apoptosis - related protein Caspase - 3. Results The MTT results showed that FIT could inhibited the growth of EC9706 cell,in which the inhibition effect was the most significant in the group treated with 60 μM FIT for 72 h. There was dependence between drug concentration and time. The adhesion experiment results showed that after cultivation for 72 h, the adhesive capacity of EC9706 cell could be significantly.weakened,and there was an effect of dependence on concentration. Western blot results showed that after cultivation with PTT, the expression of Bcl - 2 could be significantly decreased, and meanwhile the expression of Caspase - 3 could be significantly increased. Conclusion PTr can effectively inhibit the growth of EC9706 cell and promote its apoptosis. Its mechanism is correlated with the activation of the Caspase - 3 expression and the inhibition of Bcl - 2 expression. PTT may be a potential new drug for the treatment of esophageal cancer.