3种复苏方法对恶性疟原虫红内期体外培养存活的影响

Three methods of reviving Plasmodium falciparum cultured in vitro

目的观察3种复苏方法对恶性疟原虫红内期体外培养存活的影响。方法采用恶性疟原虫FCCSM/YN株作为虫源,使用3种复苏液,参照体外培养方法并作改进进行复苏试验,计算红细胞原虫感染率和存活率。结果 3种复苏方法中,方法Ⅰ、Ⅱ和Ⅲ24h疟原虫存活率分别为11.32%、29.17%和40.00%;48h疟原虫存活率分别为16.39%、30.36%和46.70%;72h疟原虫存活率分别为20.59%、37.75%和57.09%;复苏方法Ⅲ恶性疟原虫存活率较高。结论复苏方法Ⅲ的复苏效果较好,适于恶性疟原虫的复苏。

Objective To observe the effects of three methods of reviving Plas＊nodium falciparum cultured in vitro. Methods The FCCSM/YN strain of P. falciparum was used. Three types of restorative solutions were used based on in vitro culturing and modification of the method of Trager and Jensen. The rate of infection with P. falciparum and P. falciparum survival rate were calculated. Results The Plasmodium survival rate （%） at 24 h was 11.3 for method I, 29.17% for method II, and 40.00% for method III. The Plasrnodiurn survival rate （%） at 48 h was 16. 390/00 for method 1, 30.36% for method II, and 46.70% for method III. The Plasmodiurn survival rate （%） at 72 h was 20. 59% for method I, 37.75% for method II, and 57.09% for method III. P. falciparum had the highest survival rate with method IlL Conclusion Revival method III was most effective and is suitable for reviving P. falciparum.