白天挤粘法检测在校大学生蠕形螨感染效果分析

Analysis of Demodex infection among college students using skin scrapings collected with cellophane tape during the daytime

目的分析白天挤粘法检出蠕形螨感染的情况。方法采用挤粘法对434名在校大学生进行蠕形螨检查,在显微成像系统下观察并记录拍照,同时调查受检者个人卫生习惯。结果 434名学生中158人感染蠕形螨,感染率为36.41%,男生感染率为26.79%,女生感染率为42.48%,女生显著高于男生(χ2=9.64,P<0.01);毛囊蠕形螨面部感染率(69.62%)高于皮脂蠕形螨(17.09%)(χ2=44.38,P<0.01);卵检出率19.60%,高于常规检查方法的卵检出率。检出的螨虫在透明胶纸上保存时间较长,20d左右蠕形螨的形态依然清晰可辨。结论白天挤粘法取材方便快速,操作简单,观察标本时间足够,适合临床检验和大样本人群普查。

Objective To analyze the incidence of Demodex infection using skin scrapings collected with cellophane tape during the daytime. Methods Four hundred and thirty-four medical students were tested for Demodex mite infection using skin scraping and the cellophane tape method. Samples were observed and photographed with a microscopic imaging system. Health habits of the students were investigated via a questionnaire. Results The total rate of infection was 36.4 %. The infection rate for boys was 26.79% and that for girls was 42.48%. Boys had a higher infection rate than girls （X2=9.64,P〈0.01）. The rate of infection with D. folliculorurn （69.62%）was higher than that with D. brevis （17.09%）（X2=44. 38,P〈0. 01）. The rate of ova detection was 19.6%. This is higher than the rate of ova detection by a routine examination. Mites on transparent adhesive tape can be preserved for a relatively long period of time, and Demodex mites are still evident after about 20 days. Conclusion Skin scrapings collected with cellophane tape during the daytime are a convenient and fast method of detecting Demodex. This method is easy to perform and specimens can be viewed for a sufficient amount of time. This method is suitable for clinical testing and surveys of large populations.