常见三种类型胆结石胆汁中细菌群落的检测

Metagenomic analysis reveals gallstone types associated with gut microbiome community composition

目的探讨常见三种类型胆结石胆汁中所含微生物群落的差异性。方法采用末端限制性片断长度多态性(T-RFLP)技术对50例三种类型胆结石患者的胆汁细菌群落进行基因序列分析。结果 (1)细菌16S rDNA基因片段的阳性检出率为76%(38/50),三组之间阳性检出率差异无统计学意义;(2)三组细菌16S rDNA基因片段分析,纯胆固醇组的细菌群落主要以肺炎克雷伯氏菌和金黄色葡萄球菌为主;胆色素结石组主要包括普通变形杆菌、厌氧消化球菌等;混合性结石胆汁组主要为厌氧消化球菌、丙酸杆菌,其中还包括了胆色素结石胆汁组中未检测到的黄微杆菌和希氏短杆菌。结论胆结石患者胆汁标本中细菌检出率较高,但各组细菌群落构成各有不同,且成分较为复杂。

Objective To determine if the types of gallstones are associated with different bacterial profiles in bile.Methods Gallstones and bile samples were obtained from 50 patients undergoing elective cholecystectomy.Gallstone chemical composition was determined by infrared spectrometry analysis using KBr tablets.The bacteria composition of corresponding bile samples were analyzed by terminal restriction fragment length polymorphism（T-RFLP） genetic sequencing of 16s ribosomal DNA（rDNA）.Results Three types of gallstones were detected and the patients were grouped accordingly： pure cholesterol stones（n=25 patients）,pigment gallstones（n=11）,and mixed stones（n=14）.The overall positive detection rate of bacterial 16S rDNA gene fragments was 86%（43/50）,and the positive rate was not significantly different among the three groups.The microbiome of the pure cholesterol group was mainly composed of Klebsiella pneumoniae and Staphylococcus aureus.The bile pigment stones group was mainly composed of Proteus vulgaris.The mixed group was mainly composed of anaerobic digestion bacteria propionic acid bacteria,and Microbacterium flavum.Conclusion Different types of gallstones were associated with different bile microbiome profiles.