超声酶法提取百合总皂苷的研究

Study on extraction of total saponins from Lilium brownii with combined ultrasonic and enzymolysis

将超声法与酶解法相结合提取百合中总皂苷,用正交试验法对百合总皂苷提取工艺中的酶解时间、酶解pH、酶添加量、超声时间等4个因素进行研究,得出最佳且适合大规模生产的总皂苷提取工艺条件:pH4.5、酶解时间3h、酶解温度50℃、果胶酶使用量15U/g、超声时间2h、8倍加水量。在此最佳工艺条件下,百合皂苷提取量为10.45%,提取物得率为37.86%,该方法明显优于其他提取方法。

With combined ultrasonic and enzymolysis, total saponins were extracted from Lilium brownii. Orthogonal experiments were conducted to study the 4 factors - enzymolysis time, pH, dosage of pectinase and ultrasonic time - of the extraction. The optimum extraction parameters suitable for mass production were determined as： pH 4.5, enzymolysis time 3 h, enzymolysis temperature 50~C, the dosage of pectinase 15 U/g, ultrasonic time 2 h and material-water ratio 1 ： 8. On the conditions mentioned above, the extraction volume of Lilium brownii saponins was 10.45% and the extract yield 37.86%, which showed that this extraction method was evidently superior to the others.