3种体细胞共培养体系对昆明鼠早期胚胎体外发育潜力的影响研究

Effect of co-culture systems of three kinds of somatic cells on in vitro development potential of mouse pronuclear embryos

目的:评价不同体细胞共培养体系对昆明鼠早期胚胎体外发育潜力的影响。方法:采用人卵丘细胞、昆明鼠卵丘细胞、昆明鼠成纤维细胞3种共培养体系与昆明鼠2细胞期胚胎共培养,与对照组比较,观察胚胎发育情况,计数囊胚细胞数、囊胚细胞凋亡数。结果:培养24～48h,4～8细胞及桑葚胚形成率共培养3组与对照组间无差异(P>0.05);培养72h,共培养3组间扩张囊胚形成率和囊胚形成率无差异(P>0.05),但均明显高于对照组(P<0.05);共培养3组间囊胚细胞数及囊胚细胞凋亡率无差异(P>0.05),但与对照组比较存在差异(P<0.05)。结论:不同类型的体细胞共培养体系均可在体外为胚胎发育提供一种更类似于体内的环境,有利于胚胎在体外发育。

Objective： To explore the effect of different kinds of somatic cell co - culture systems on in vitro development of mouse early embryos. Methods： Kunming mouse 2 - cell embryos were cuhured to blastocysts with co - culture systems of three kinds of somatic cells （human cumulus cells, Kunming mouse cumulus cells and Kunming mouse fibroblasts）. Blastocyst formation rates, cell numbers and apoptotic rates were compared with the control group. Results ： After 24 to 48 hours of culture in vitro, differences in formation rates of 4 - to 8 - cell embryos and morulas were not found between the co - culture systems group and the control group （P 〉 0.05）. After 72 hours of culture in vitro, there were no diffecences in the formation rates of expanded blastocysts and blastocysts among three groups of co - culture systems （ P 〉 0.05 ）, while which were significantly higher than those of the control group. There were no differences in mean cell number and the apoptotic rate of blastocysts among three groups of co - cluture systems （P 〉 O. 05）. But there were significant differences between the co - culture systems group and the control group （ P 〈 0.05 ）. Conclusion ： Different types of somatic cell co - culture systems may provide an in vitro environment similar with in vivo environment for embryo culture, which facilitate the in vitro development of embryos.