rAd-p53瘤内注射联合放化疗前后鼻咽癌原发灶细胞凋亡的变化

目的观察重组人p53腺病毒(rAd-p53)瘤内注射联合放化疗前后的鼻咽癌原发灶组织细胞凋亡的变化情况。方法 61例确诊中晚期鼻咽癌的患者随机分为2组。治疗组:rAd-p53瘤内注射+同步放化疗。对照组:同步放化疗组。于rAd-p53瘤内注射前及放疗至20G(y第三次rAd-p53治疗后)时取鼻咽部瘤体活组织标本,分别进行凋亡细胞的原位检测(TUNEL法)。结果对照组治疗前后的AI分别为(4.455±1.670)%、(8.252±4.076)%,治疗组治疗前后的AI分别为(4.453±1.670)%、(13.370±6.957)%,治疗组治疗前后、对照组治疗前后、治疗组与对照组治疗后AI有统计学差异(P<0.05)。结论 rAd-p53瘤内注射联合放化疗鼻咽癌原发灶细胞凋亡指数明显增高。

Objective To compare the changes of apoptosis after adenovirus p53 （rAd-p53） intratumoral injection combined with radiotherapy and chemotherapy on treatment of advanced stage nasopharyngeal carcinoma. Methods 61 cases with advanced stage nasopharyngeal carcinoma were randomly divided into two groups. The therapy group： intratumoral injection rAd-p53 plus radiotherapy and chemotherapy group, the control group： radiotherapy and chemotherapy group. The histopathological changes were compared between the two groups. All samples taken apoptotic examination by in situ TUNEL. Result^Before and after treatment, the apoptotic index of therapy group was （4.453±1.670）% and （13.370±6.957）% respectively, the apoptotic index of the control group was （4.455±1.670）% and （8.252±4.076）% respectively. The apoptotic index of therapy group was higher than the the control group after treatment （P〈0.05）.Conclusion Apoptotic index in nasopharyngeal carcinoma was a remarkable increase after adenovirus p53 （rAd-p53） intratumoral injection combined with radiotherapy and chemotherapy.