外源激素对紫穗狼尾草愈伤组织诱导及分化的影响

Effects of external hormones on induction and differentiation of callus of Chinese pennisetum

以紫穗狼尾草(Pennisetum alopecuroides)幼芽基部为外植体,研究了外源激素对其愈伤组织诱导及分化的影响,筛选适宜的激素种类及浓度配比,为狼尾草无性系抗性育种奠定基础。结果表明,2,4-D对愈伤组织诱导的作用最显著,生长素NAA和KT的作用相近,均以0.5～1.0mg.L-1为宜;NAA对愈伤组织分化的作用较明显,以0.5～1.0mg.L-1与3.0mg.L-16-BA配合效果最佳,KT的作用不明显。最佳诱导培养基为MS+2,4-D 3.0mg.L-1+NAA 1.0mg.L-1+KT 1.0mg.L-1;最佳分化培养基为MS+NAA 0.5mg.L-1+6-BA 3.0mg.L-1。

The base of young shoots of Pennisetum alopecuroides was used as explants in this experiment to determine the effect of external hormones on induction and differentiation of callu：3 of Chinese pennisetum by screening appropriate hormone type and concentration ratio. The results of this study showed that the 2, 4-D was significantly effective for callus induction. The effect of hormone NAA and KT on growth was similar and their appropriate concentration was 0.5-1.0 mg L^-1. The NAA was effectively useful for cal- lus differentiation under combination 0.5-1.0 mg·L^-1 NAA and 3.0 mg·L^-1 6-BA, but KT did not work on callus differentiation. This study suggested that the best induction cukure medium was MS＋ 2, 4-D 3.0 mg·L^1＋NAA 1.0 mg·L^-1＋KT 1.0 mg·L^-1 , and the best differentiation culture medium was MS＋NAA 0.5 mg·L^-1 ＋6-BA 3.0 mg·L^-1 , which would provide information for resistance breed- ing of Penniseturn clones.