一株溶藻菌的分离及其溶藻特性分析

Isolation and Analysis of an Algae-Lysing Bacteria

[目的]为微生物溶藻工程的实施提供较好的材料。[方法]以淮北水华严重地点作为取样点,筛选到溶藻效果明显的一菌株Q6,通过显微观察和叶绿素a下降率的测定等方法,研究其溶藻效果。[结果]该菌无菌滤液作用3 d,使藻培养液出现黄化,叶绿素a的下降率达70%;5 d时泛白,叶绿素a的下降率达85.6%;Q6菌株无菌滤液具有明显的溶藻效果,证实Q6是通过分泌胞外物质进行溶藻的。经高温、蛋白酶和乙醇处理后的上清液均能溶解水华鱼腥藻,溶藻物质应为具有热稳定性的非蛋白类、核酸和糖类等物质,推测为某种抗生素。[结论]该菌来源于池塘,菌体呈环形,革兰氏染色阴性,菌落有浅玫瑰红色,结合形态观察和生理生化特性分析,初步鉴定Q6为微环菌属。

[Objective]The aim was to provide the better material for implementation of Algae-Lysing engineering.[Method]An algae-lysing strain Q6 was isolated from the lake of Huaibei city,which was capable to solute the algae.The effect of algal lysis was monitored by chlorophyll-a measurement and microscopy.[Result]Results showed that the Q6 had a good removal effect on Anabaena flosaquae： Algal culture contained bacteria-free filtrate of Q6 appeared yellow after three days,the decline rate of chlorophyll-a was 70%;Algal culture contained bacteria-free filtrate of Q6 appeared white after five days,the decline rate of chlorophyll-a was 85.6%,which indicated that extracellular lytic substance produced by Q6 cells could have lytic effect on algae.The experiment showed that Q6 lysed Anabaena flosaquae by excreting an extracellular product which mainly consisted of thermo-stable non-protein,non-nucleic acid,non-polyhexose substances.[Conclusion]The Q6 was identified as Microcyclus genus based on morphology and physiological biochemistry characteristic analysis.