高表达粟酒裂殖酵母SpTrz2p对细胞周期的影响

Effect in Cell Cycle Caused by Overexpression of SpTrz2p in Schizosaccharomyces pombe

[目的]测定粟酒裂殖酵母高表达的SpTrz2p对其细胞周期的影响。[方法]将粟酒裂殖酵母的trz2+克隆到pREP4x质粒上,构建带有SpTrz2的高表达载体,将构建成功的重组子转化至野生型粟酒裂殖酵母细胞中,利用流式细胞仪检测细胞周期的变化。[结果]高表达SpTrz2p可导致酵母细胞形态和周期的改变,使大部分酵母细胞停留于G1期,这说明高表达SpTrz2p对细胞周期有明显影响。[结论]该研究结果表明高表达SpTrz2p给细胞造成的致死毒性是通过影响细胞周期实现的。

[Objective] This study aimed to investigate the effect in cell cycle caused by overexpression of SpTrz2p in Schizosaccharomyces pombe.[Method] The sptrz^2＋ gene from S.pombe was cloned into pREP4x plasmid to construct an overexpression vector of SpTrz2p,which was then transformed into wild-type S.pombe cells.The cell cycle was determined with flow cytometry.[Result] Overexpression of SpTrz2p in yeast caused changes in cell morphology and cell cycle.The majority of the cells were arrested at G1 phase,indicating that overexpression of SpTrz2p indeed affected the cell cycle.[Conclusion] This study suggested that overexpression of SpTrz2p is lethal to the cells by affecting the cell cycle.