新疆野核桃叶提取物抑菌试验研究

Study on Bacteriostasis Test of Xinjiang Wild Walnut Leave Extract

试验选用新疆野核桃叶为研究对象,以金黄色葡萄球菌为指示菌,用抑菌圈直径为指标,在单因素试验的基础上通过正交实验对核桃叶的提取条件进行优化。结果表明:对核桃叶抑菌活性物质提取效果的影响为乙醇浓度>液固比>提取温度>提取时间;最佳提取条件:乙醇浓度70%,液固比15∶1,提取温度60℃,提取时间4h。在此优化条件下,核桃叶提取物抑菌圈直径为1.932cm。

This experiment through wild walnut leaf extract, confirmed the vitro antimicrobial test of wild walnut leaf extract gram- positive bacteria have obvious antibacterial function. Xinjiang wild walnut leaf test for researching object,by staphy- lococcus aureus, with instructions for the bacteriostatic circle diameter of activity tracking, on the basis of single factor experi- ment through orthogonal experiment of walnut leaf extract optimized conditions. As Results of walnut leaves the antibacterial activity material extraction effect size order： Ethanol concentration 〉 The liquid - solid ratio 〉 Extracting temperature 〉 Ex- tracting time;The best condition： ethanol concentration70%, The liquid- solid ratio15： 1, Extracting temperature60℃, Ex- tracting time 4 hours o In the optimized conditions, walnut leaf extract bacteriostatic circle for 1. 932 cm in diameter.