儿童非特异性慢性咳嗽病因及与TRPV1基因多态性的关系

Etiology of nonspecific chronic cough in children and relationship between TRPV1 gene polymorphisms and nonspecific chronic cough

目的研究儿童非特异性慢性咳嗽病因及与瞬时感受器离子通道受体1(TRPV1)基因多态性的关系。方法对195例非特异性慢性咳嗽患儿首次就诊后半个月、1个月、3个月进行随访,以明确病因。采用聚合酶链反应限制性酶切片段多态性(PCR-RFLP)方法对其进行TRPV1基因rs222747、rs222748、rs8065080位点的基因型和等位基因分析。205例健康或无慢性咳嗽的外科患儿作为对照。结果 195例非特异性慢性咳嗽患儿病因分布如下:咳嗽变异性哮喘(CVA)96例(49.2%)、CAV合并上气道咳嗽综合征(CVA+UACS)48例(24.6%)、感染后咳嗽34例(17.4%)、上气道咳嗽综合征(UACS)17例(8.7%)。TPPV1基因rs222747、rs222748、rs8065080位点均可检出3种基因型:rs222747(CC、GC、GG);rs222748(CC、TC、TT);rs8065080(CC、TC、TT)。rs222747位点基因多态性分布不符合Hardy-Weinberg定律,故未进行下一步分析。慢性咳嗽组和对照组比较rs222748位点、rs8065080位点基因型和等位基因频率经Bonferroni多重检验校正差异均无统计学意义。结论非特异性慢性咳嗽患儿中CVA、UACS、感染后咳嗽是引起儿童慢性咳嗽的主要病因。TRPV1基因rs222748和rs8065080位点基因多态性可能与非特异性慢性咳嗽的发生无关。

Objective To explore the causes of nonspecific chronic cough in children and relationship between transient receptor potential vanilloid 1 （ TRPV1 ） gene polymorphisms and nonspecific chronic cough. Methods A total of 195 children with chronic cough were followed up half a month, one month and three months after their first visit to hospital. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to examine polymorphisms of the TRPV1 gene in the children. A total of 205 healthy or surgical children without chronic cough served as the control group. Results The etiologic distribution of the 195 children with chronic cough was as follows： 96 （49.2%） cases of cough variant asthma （ CVA ）, 48 （ 24. 6% ） cases of CVA complicated by upper airway cough syndrome （ UACS）, 34 （ 17.4% ） cases of post-infectious cough, and 17 （8.7%） cases of UACS. Three genotypes were identified in both groups at positions rs222747 （ CC, GC and GG）, rs222748 （ CC, TC and Tr） and rs8065080 （ CC, TC and TY）. The frequencies of genotype and allele at position rs222747 did not accord with the law of Hardy-Weinberg. There was no significant difference in frequencies of genotype and allele at positions rs222748 and rs8065080 between the two groups. Conclusions CVA, UACS and post-infectious cough are common causes of nonspeeifie chronic cough in children. TRPV1 gene polymorphisms at positions rs222748 and rs8065080 may be unrelated to nonspecific chronic cough in children.