哈氏噬纤维菌吸附纤维素的影响因素

Factors affecting adhesion of Cytophaga hutchinsonii to cellulose

【目的】探索哈氏噬纤维菌(Cytophaga hutchinsonii)吸附纤维素的作用机制。【方法】通过比较不同因素对哈氏噬纤维菌吸附纤维素的影响,包括:菌龄、pH、温度、表面电荷、细胞活力、细胞表面蛋白、细胞表面多糖以及纤维素类似物等,寻找在吸附过程中起重要作用的细胞成分。【结果】菌体经蛋白酶及热处理,对纤维素的吸附能力完全丧失;叠氮化钠、甲醛和戊二醛处理对菌体吸附能力影响不明显;菌体经刚果红和高碘酸钠处理,吸附能力变化不大;菌体对纤维素底物的吸附具有特异性,吸附作用不受纤维二糖和羧甲基纤维素的抑制。【结论】实验表明,哈氏噬纤维菌吸附纤维素的能力与菌体表面蛋白密切相关,而受细胞的代谢活性和胞外多糖影响较小,推测细胞表面可能存在特异性的纤维素结合蛋白。

[Objective]The aim of the study was to understand the mechanism of Cytophaga hutchinsonii adhension to cellulose.[Methods] The effects of different factors on the bacterial adhesion to cellulose were studied,including bacterial age,pH,temperature,cell surface charge,cell viability,cell surface protein,extracellular polysaccharides,and cellulose derivates.[Results] Treatments with heat and protease reduced the adhesion remarkably.But treatments with NaN3,formalin,glutaraldehyde,Congo red and NaIO4 had only slight effect on the adhesion.The adhension of Cytophaga hutchinsonii cells to microcrystalline cellulose was specific and not inhibited by cellobiose or carboxymethyl cellulose.[Conclusion] The adhesion of Cytophaga hutchinsonii to cellulose was closely related to cell surface proteins,while cellular metabolic activity and extracellular polysaccharides had only slight effect on it.It is speculated that there might be some specific cellulose binding proteins on the cell surface.