槲皮素对人肾癌细胞株细胞增殖、侵袭及其VEGF表达的影响

Effects of quercetin on proliferation,invasion and VEGF protein expression on human renal cell cancer of ACHN cell line

目的研究槲皮素对人肾癌细胞株(ACHN)细胞增殖、侵袭力的影响,并探讨其可能机制。方法体外培养ACHN细胞,加入不同浓度的槲皮素进行处理后,用MTT比色分析法检测细胞增殖抑制率,采用涂有Matrigel胶的Tr-answell小室模型对传代培养的ACHN细胞进行体外侵袭检测,以Western blot法检测ACHN细胞内血管内皮生长因子(VEGF)的表达。结果不同浓度的槲皮素均能抑制ACHN细胞的增殖,并有明显的浓度和时间依赖性(P<0.05);Tr-answell试验结果显示,与空白对照组比较,槲皮素处理组肾癌细胞侵袭能力明显下降;槲皮素处理组VEGF蛋白表达水平均明显低于空白对照组,且呈浓度依赖性。结论槲皮素可明显抑制ACHN细胞的增殖、侵袭力,其机制可能与下调VEGF表达有关。

Objective To study the effect of quercetin on the proliferation and invasion on human renal cell cancer of ACHN cell line,and investigate the probable molecular mechanisms.Methods Different concentrations of quercetin were used to treat ACHN cells cultured in vitro,the inhibition of proliferation of ACHN cell was studied by methyl thiazolyl tetrazolium（MTT） assay.The abilities of cell invasion was evaluated by Transwell chambers,and the expression of vascular endothelial growth factor（VEGF） was detected by Western blot analysis.Results After quercetin treatment,the proliferation and invasive ability of ACHN cells were obviously decreased,which showed a dose-and time-dependent manner（P 〈 0.05）.Meanwhile,quercetin could down-regulate the expression of VEGF protein in a dose-dependent manner.Conclusion Quercetin can inhibit the proliferation and invasion of ACHN cells by down-regulating VEGF expression.