摘要
目的观察1,25-二羟维生素D3对树突状细胞(DC)的表型、功能以及对其Toll样受体7(TLR7)表达的影响,探讨TLR7在1,25-二羟维生素D3诱导耐受性DC可能的机制。方法体外扩增小鼠骨髓来源的DC,采用流式细胞术和混合淋巴细胞反应检测1,25-二羟维生素D3对DC表型和功能的影响;利用RT-PCR半定量方法测定1,25-二羟维生素D3对DC的TLR7表达的影响。结果 1,25-二羟维生素D3处理后,DC表达CD86和MHC II的荧光强度均明显降低,差异有统计学意义(P<0.05);对T细胞的刺激能力较对照组明显减弱(P<0.05);与对照组比较,1,25-二羟维生素D3可以明显下调TLR7的表达(P<0.01)。结论 1,25-二羟维生素D3可能通过下调TLR7的表达抑制DC细胞的成熟,从而使得DC具有耐受性特征。
Objective To investigate the influence of 1,25-dihydroxyvitamin D3 on dendritic cell(DC) of phenotype,function and TLR7 expression,to explore the possible role of TLR7 in the procedure of the inducement of tolerance DC by 1,25-dihydroxyvitamin D3.Methods DC were harvested by differentiating mouse borrow cells in vitro.The influence of 1,25-dihydroxyvitamin D3 on phenotype and function of DC was determined by flow cytometry and mixed lymphocyte reactions.The expression of TLR7 on 1,25-dihydroxyvitamin D3-induced DC was determined by semi-quantitative RT-PCR.Results Treated by 1,25-dihydroxyvitamin D3,DC had significantly lower fluorescence intensity of CD80 and MHCⅡ(P〈0.05),and DC ability to stimulate T cells was significantly lower than that of the control(P〈0.05).Compared to the control,1,25-dihydroxyvitamin D3 could obviously down-regulate the expression of TLR7(P〈0.01).Conclusion 1,25-dihydroxyvitamin D3 can inhibit the maturation of DC,and may make DC tolerant by down-regulating the expression of TLR7.
出处
《安徽医科大学学报》
CAS
北大核心
2012年第8期924-927,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金项目(编号:1208085MH154)