TRAF3在肝细胞肝癌中的表达及意义

Significance of TRAF3 protein expression in hepatocellular carcinoma

目的探讨肿瘤坏死因子受体相关因子3(TRAF3)在肝细胞癌(简称肝癌)发生、发展中的意义。方法采用免疫组化方法检测50例原发性肝癌组织、12例癌旁肝组织及10例正常肝组织中TRAF3蛋白的表达,分析其与肝癌的临床病理参数的关系。Western blot法检测TRAF3在肝癌高分化细胞系HepG2、低分化细胞系SMMC7721和人正常肝细胞系L-02中的表达差异。结果 TRAF3主要定位于细胞质,其阳性率在正常肝组织(100%)及癌旁组织(91%)明显高于肝细胞癌组织(52%)。TRAF3在肝癌的表达与临床分期相关,与患者性别、年龄、癌灶数目、肿瘤大小、瘤栓有无、乙肝表面抗原(HBsAg)阴阳性、甲胎蛋白(AFP)、肿瘤包膜是否完整均无关。TRAF3总蛋白在正常肝细胞系中的表达量明显高于肝癌细胞系,且在肝癌高分化的细胞系中表达量高于低分化细胞系。结论 TRAF3在肝癌中的表达明显下调,且在肝癌中的表达水平与肝癌的侵袭程度相关,提示TRAF3可能抑制肝癌的浸润和转移。

Objective To study the significance of tumor necrosis factor receptor associated factor 3（TRAF3） in the carcinogenesis and development of hepatocellular carcinoma（HCC）.Methods The expressions of TRAF3 in 50 HCC specimens,12 carcinoma tissue specimens and 10 normal hepatic tissue specimens were detected by immunohistochemistry.The expressions of TRAF3 in HCC well-differentiated cells HepG2,poorly differentiated cells SMMC7721 and human normal liver cells L-02 were detected by Western blot.Results TRAF3 was mainly expressed in cytoplasm.The positive rate of TRAF3 in normal liver tissues（100%） and adjacent normal tissues（91%） were significantly higher than that in HCC（52%）.Expression of TRAF3 was related to clinical stage,while not related to sexuality,age,the number of cancers,size,tumor embolus,HBsAg,AFP and tumor capsule.The protein level of TRAF3 was obviously higher in L-02 normal liver cells than that in HCC and it was slightly higher in HepG2 cells than that in SMMC7721 cells.Conclusion The expression of TRAF3 in HCC is suppressed,and correlative to the invasive ability of HCC.These results suggest that TRAF3 can inhibit invasion and metastasis in HCC.