风湿性二尖瓣狭窄瓣膜组织CCL19和CCR7的表达

Analysis of CCL19 and CCR7 expressions in mitral valves in patients with rheumatic mitral stenosis

目的研究趋化因子配体19(CCL19)及其受体趋化因子受体7(CCR7)在风湿性二尖瓣狭窄(RMS)患者瓣膜组织中的表达、分布特点和细胞定位。方法采用GeneChip Human Gene1.0ST基因芯片和免疫组织化学染色法检测RMS病变二尖瓣瓣膜(A组,16例)和正常瓣膜(B组,3例)中CCL19和CCR7的表达情况。结果 A组CCL19和CCR7均高表达;其中,CCL19主要分布在炎症细胞区域,CCR7主要在瓣膜间质细胞表达。而B组中CCL19和CCR7均无明显表达。结论 RMS患者二尖瓣瓣膜组织中CCL19主要表达于浸润的炎症细胞,CCR7表达于瓣膜间质细胞,两者相互作用参与瓣膜的病理过程。

Objective To investigate the expressions,distributions and cellular locations of C-C chemokine ligand 19（CCL19） and C-C chemokine receptor 7（CCR7） in mitral valve tissues of patients with rheumatic mitral stenosis（RMS）. Methods The expressions of CCL19 and CCR7 in mitral valves from 16 RMS cases （group A） and 3 normal subjects（group B） were detected by GeneChip Human Gene 1.0 ST array and immunohistochemistry. Results CCL19 and CCR7 were highly expressed in group A. CCL19 was detected in the infiltrated area of inflammatory cells, and CCR7 was mainly expressed in interstitial cells. However, neither CCL19 nor CCR7 was obviously found in group 13. Conclusion CCL19 in mitral valve tissues of RMS patients is mainly expressed in inflammatory cells, while CCR7 locates in interstitial cells. Interaction between CCL19 and CCR7 is involved in the pathological process of RMS.