摘要
目的研究p53正向细胞凋亡调控因子(PUMA)基因mRNA及其功能蛋白在肝癌组织中的表达及其临床意义。方法用半定量RT-PCR和Western blot方法检测42例肝癌患者的癌组织及其癌旁组织和42肝良性病变组织的PUMA mRNA及其编码蛋白表达。结果癌组织、癌旁组织和肝良性病变PUMA mRNA的表达量分别为0.81±0.07、0.97±0.04和1.76±0.06,PUMA蛋白的表达量分别为0.48±0.19、0.61±0.12和1.11±0.34;肝癌组织及癌旁组织的PUMAmRNA及其蛋白表达量明显低于肝良性病变组织高于(P<0.05)。结论 PUMA基因在肝癌组织中的表达下调,与癌的发生呈负相关。
Objective To explore the expression of p53 up-regulated modulator of apoptosis （PUMA） gene in hepatocellular carcinoma （HCC）. Methods With semi-quantitative RT-PCR and Western blot, the expressions of PUMA mRNA and protein were detected in 42 HCC tissues（group A） ,tumor-adjacent tissues（group B） and 42 benign liver tissues（group C）. Results The expressions of PUMA mRNA in groups of A,B and C were 0.81±0.07,0.97±0.04, and 1.76±0.06, respectively,which of PUMA protein were 0.48±0. 19,0.61±0.12 and 1.11±0.34. The expressions of PUMA mRNA and its protein were lower in groups of A and B than those in group C（P〈0. 05）. Conclusion The expression of PUMA gene in HCC tissues is down-regulated, which is negatively correlated with the development of liver cancer.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第14期1673-1675,共3页
Jiangsu Medical Journal
关键词
p53正向细胞凋亡调控因子
肝癌
p53 Up-regulated modulator of apoptosis
Hepatocellular carcinoma
