2-(2′-吡啶基)苯并噁唑和甘氨酸铜配合物的结构、抑菌活性及与DNA作用

Structure,Antibacterial Activities and DNA Interaction of a Copper(Ⅱ) Complex with 2-(2′-Pyridyl) benzoxazole and Glycinate

本文以2-(2′-吡啶基)苯并噁唑、甘氨酸和高氯酸铜髤为原料合成了新的三元配合物:[Cu(PBO)(Gly)(H2O)]ClO4(PBO=2-(2′-吡啶基)苯并噁唑,Gly=甘氨酸根)。应用元素分析、红外光谱、紫外可见光谱、摩尔电导率等方法对配合物进行了表征。X-射线单晶衍射测得该配合物晶体属正交晶系,Pca21空间群,晶胞参数:a=1.029 2(2)nm,b=1.032 9(2)nm,c=1.566 8(3)nm;Z=4,V=1.665 6(6)nm3,Dc=1.800 g.cm-3,F(000)=916。最终偏离因子:R1=0.042 6,wR2=0.101 3。利用试管二倍稀释法测定了配合物的抗菌活性,通过电子吸收光谱、荧光光谱和粘度测定研究了配合物与DNA的作用。结果表明,与2-(2′-吡啶基)苯并噁唑相比较,该配合物对枯草杆菌(B.subtilis,G+),金黄色葡萄球菌(S.aureus,G+),苏云金芽孢杆菌(B.thuringiensis,G+),水稻条斑病细菌(X.oryzae,G-),大肠杆菌(E.coil,G-)和沙门氏杆菌(Salmonella,G-)具有良好的抑制活性,并且以插入方式与DNA作用。

A new ternary copper（Ⅱ） complex： [Cu（PBO）（Gly）（H2O）]ClO4（PBO=2-（2′-pyridyl） benzoxazole,Gly=glycinate）,was synthesized and characterized by elemental analysis,molar conductivity,IR,UV-Vis and single crystal X-ray diffraction.The crystal belongs to orthorhombic,space group Pca21,with the crystal cell parameters： a=1.029 2（2） nm,b=1.032 9（2） nm,c=1.566 8（3） nm,Z=4,V=1.665 6（6） nm3,Dc=1.800 g·cm-3,F（000）=916,R1=0.042 6,wR2=0.101 3.The complex was assayed against gram-positive（S.aureus,B.subtilis,B.thuringiensis） and gram-negative（X.oryzae,Salmonella,E.coil） bacteria by doubling dilutions method,and the interaction of the complex to DNA was investigated by electronic absorption spectroscopy,fluorescence spectroscopy,and viscosity measurement.The results show that the complex has good antibacterial activities and can bind to DNA by intercalative mode.CCDC： 852991.