阿魏酸过渡金属配合物的合成及抗凝血作用研究

Synthesis and Anticoagulant Properties of Transition Metal Complexes with Ferulic Acid

用阿魏酸与Fe、Co、Ni、Cu、Zn的硝酸盐制备了5种过渡金属配合物,通过红外光谱、热重-差热分析、元素分析、荧光和紫外光谱的方法对配合物进行了表征,确定了配合物的组成。并对配体和配合物进行了全血凝血时间、复钙时间、活化部分凝血活酶时间、凝血酶原时间的测定,结果表明5种配合物具有较好的抗凝血性质。通过荧光光谱、紫外光谱、圆二色谱研究了配合物与人血清白蛋白(HSA)的相互作用,HSA的荧光光谱表明配合物对其有荧光猝灭作用并使其发射峰位置红移,紫外光谱表明配合物的加入导致HSA吸收强度增加且吸收峰位置紫移,圆二色谱表明配合物的存在可引起HSA构象的变化。推测配合物抗凝血作用的起效与其和血清白蛋白之间的相互作用有一定的联系。

The complexes of（C10H9O4）xM·mH2O（M=Fe,Co,Ni,Cu,Zn） were obtained by the reaction of ferulic acid with metal ions in ethanol solution by solvent-thermal method.The composition of this complexes were characterized by IR,UV,fluorescence spectra,TG-DTA and elemental analysis methods.Meanwhile,the anticoagulant properties of ferulic acid and its complexes were tested by coagulation time（CT）,recalcification time（RT）,activated partial thromboplastin time（APTT）,and prothrombin time（PT）.The results showed that the complexes all have better anticoagulant action than that of ferulic acid in related anticoagulant experiments.It was observed that ferulic acid and the complexes could reduce the fluorescence intensity and enhanced the UV intensity of human serum albumin（HSA）.The precense of complexes can cause a change of the human serum albumin conformation.The interaction between HSA and the complexes may be the one of reasons for anticoagulant function of the complexes.