C57BL／6J及NIH Swiss小鼠在抑郁模型中的行为学表现

Behavioral manifestations in animal models with depression between C57BL/6J and NIH Swiss mice

目的探讨两种不同遗传背景的小鼠C57BL／6J及NIH Swiss在抑郁模型中的行为学差异，为抗抑郁药物研究的实验动物选择提供参考，为研究遗传学因素在抑郁症发病过程中的作用提供有用的动物模型。方法C57BL／6J、NIH Swiss小鼠各14只，利用强迫游泳实验和悬尾实验检测两种小鼠在应激条件下的抑郁样行为表现；利用旷场实验检测两种小鼠的自主运动能力。结果强迫游泳实验中，C57BL／6J小鼠的不动时间明显长于NIH Swiss小鼠[（133．198±5．749）s比（80．265±10．939）s，P=0．000]。悬尾实验中，C57BL／6J小鼠的不动时间高于NIH Swiss小鼠[（151．315±12．161）s比（95．107±14．649）s，P=0．007]。而在旷场实验中，两种品系的小鼠运动总路程、站立次数、运动时间、边缘区运动路程、边缘区运动时间、中央区运动路程、中央区运动时间差异均无统计学意义。结论C57BL／6J小鼠在急性应激的情况下更容易表现出抑郁样行为，而NIH Swiss小鼠则表现出一定的抑郁抗性，两种不同品系小鼠的不同行为学表现可能由于遗传因素导致。

Objective To investigate behavioral differences in models with depression between C57BL/6J and NIH Swiss mice with different genetic backgrounds, to provide references for selection of examination animals in anti- depression drug researches, and to provide the useful animal models for investigating the effects of genetic factors on occurrence of depression. Methods Behavioral manifestations of depression in C57BL/6J and NIH Swiss mice （n=14 each） were detected under stress conditions using forced swimming test and tail suspension test. Open field test was used to detect the spontaneous motility of two kinds of mice. Results The immobility time of C57BL/6J mice was significantly longer than that of NIH Swiss mice [ （133.198±5.749） s vs （80.265±10.939） s, P=0.000] in forced swimming test, which was contrast to that in tail suspension test [ （151.315±12.161 ） s vs （95.107±14.649） s, P=0.007]. However in open field test, no difference was found in total distance, standing number, movement time, total distance in fringe area, movement time in fringe area, total distance in central area and movement time in central area between the two kinds of mice. Conclusion C57BL/6J mice are easier to show the depressive-like behavior under stress conditions, while NIH Swiss mice show a certain of depression resistance. These different behavioral manifestations in two different kinds of mice may be caused by genetic factors.