右归丸诱导大鼠骨髓基质细胞分化为神经样细胞的研究

Study on the effect of differentiation of rat bone marrow stromal cells into neuroncyte-like cells inducing by Youguiwan

目的:探讨右归丸体外诱导大鼠骨髓基质细胞分化为神经样细胞。方法:密度梯度离心法结合贴壁培养法培养骨髓基质细胞,并利用右归丸诱导骨髓基质细胞分化为神经样细胞,设为空白对照组,右归丸组。结果:诱导7 d后,与空白对照组相比较,右归丸组细胞显示为典型的神经细胞样形态,细胞NSE、Nestin和GFAP阳性率明显增高(P<0.05)。结论:右归丸可以体外诱导大鼠骨髓基质细胞分化为神经样细胞。

Objective： To investigate differentiation of bone marrow stromal cells into neurocyte-like cells inducing by the Yougniwan. Methods： Marrow stromal cells were isolated by attachment and density centrifugation. Yougniwan were used to induce bone marrow stromal cells. Bone Marrow stromal ceils were divided into blank control group, Yougniwan group. Results： After 7 days of induction, some cell showed typical neuronal morphology, Nestin, NSE and GFAP-posi- tire expression rate was significantly higher in Yougniwan（ P 〈 0.05 ）. Conclusion： Youguiwan can induce bone marrow stromal ceils to diffenentiate into neuroncyte-like cells.