CDKN2A/2B基因多态性与妊娠糖尿病相关

Correlation of CDKN2A/2B gene single nucleotide polymorphisms with gestational diabetes mellitus

目的研究CDKN2A/2B基因rs10811661的单核苷酸多态性(SNP),探讨其与妊娠糖尿病(GDM)的相关性。方法选取鲁西南地区正常糖耐量孕妇(NGT)100例、GDM患者120例、2型糖尿病(T2DM)患者100例作为研究对象,提取基因组DNA,采用PCR-RFLP方法检测CDKN2A/2B基因rs10811661多态性。结果 CDKN2A/2B基因rs10811661的TT、TC、CC 3种基因型分布在NGT组与GDM组间有显著差别(P<0.01),GDM组危险等位基因T分布频率显著高于NGT组(P<0.05)。3种基因型及等位基因分布在NGT组与T2DM组之间亦有显著差别(P<0.05)。结论在鲁西南地区女性人群中,CDKN2A/2B基因rs10811661 T/C多态性可能与妊娠糖尿病有明显相关性。

Objective To investigate the relationship between polymorphisms of rs10811661 in CDKN2A/2B gene and gestational diabetes. Methods The 100 normal glucose tolerance pregnant women, 120 gestational diabetes patients and 100 type 2 diabetes patients living in the southwest of Shandong Province were recruited. Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, we determined the genotypes of rsl0811661 in CDKN2A/2B. Results There was significant difference in genotypic TT, TC and CC frequency of CDKN2A/2B rsl0811661 between NGT group and GDM group （P 〈 0.01 ）. The risk allele T frequency in GDM group was significant higher than that in NGT group（P 〈0. 05）. So did the difference between NGT group and T2DM group（P 〈 0.05 ）. Conclusions The rs10811661 T/C polymorphisms of CDKN2A/2B gene may be related with the gestational diabetes in the women of the southwest of Shandong Province.